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calcein am pi double staining kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/double+staining+kit/Cellstain+Double+Staining+Kit/pm42839583-421-6-10
    Average 95 stars, based on 149 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Double Staining:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.
    Article Snippet: Subsequently, the cells were cultured for an additional 72 h, after which their viability was assessed using a WST-1 assay kit (Roche Diagnostics, IN). .. Cell Viability Live/Dead Assay: For the live/dead assay, a portion of the aforementioned samples was subjected to further examination using calcein-AM and propidium iodide (PI) staining reagents obtained from the Double Staining kit (Dojindo, Japan). .. Subsequently, fluorescence microscopy (Nikon, Eclipse Ts2R) was employed to capture images of the stained samples.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Examination of Vascular Capacity of Scaffold Free Human Microtissue During Osteogenesis
    Article Snippet: .. To understand vital effects of hBMSC and HUVEC coculture, live and dead assay was performed by Double Staining Kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) [20]. ..

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We use si-GSK-3ß (D-003010-09, Horizon Discovery, Cambridge, UK) and si-control (D-001206-14, Horizon Discovery, Cambridge, UK). .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 hours after Actinonin administration.

    Staining:

    Article Title: Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.
    Article Snippet: Subsequently, the cells were cultured for an additional 72 h, after which their viability was assessed using a WST-1 assay kit (Roche Diagnostics, IN). .. Cell Viability Live/Dead Assay: For the live/dead assay, a portion of the aforementioned samples was subjected to further examination using calcein-AM and propidium iodide (PI) staining reagents obtained from the Double Staining kit (Dojindo, Japan). .. Subsequently, fluorescence microscopy (Nikon, Eclipse Ts2R) was employed to capture images of the stained samples.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We use si-GSK-3ß (D-003010-09, Horizon Discovery, Cambridge, UK) and si-control (D-001206-14, Horizon Discovery, Cambridge, UK). .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 hours after Actinonin administration.

    Cell Culture:

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Fluorescence:

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Microscopy:

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.



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