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calcein am pi double staining kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 97/100, based on 975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/double+staining+kit/Calcein/pmc13081185-70-6-13
    Average 97 stars, based on 975 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Double Staining:

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.
    Article Snippet: Subsequently, the cells were cultured for an additional 72 h, after which their viability was assessed using a WST-1 assay kit (Roche Diagnostics, IN). .. Cell Viability Live/Dead Assay: For the live/dead assay, a portion of the aforementioned samples was subjected to further examination using calcein-AM and propidium iodide (PI) staining reagents obtained from the Double Staining kit (Dojindo, Japan). .. Subsequently, fluorescence microscopy (Nikon, Eclipse Ts2R) was employed to capture images of the stained samples.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Examination of Vascular Capacity of Scaffold Free Human Microtissue During Osteogenesis
    Article Snippet: .. To understand vital effects of hBMSC and HUVEC coculture, live and dead assay was performed by Double Staining Kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) [20]. ..

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We use si-GSK-3ß (D-003010-09, Horizon Discovery, Cambridge, UK) and si-control (D-001206-14, Horizon Discovery, Cambridge, UK). .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 hours after Actinonin administration.

    Staining:

    Article Title: Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.
    Article Snippet: Subsequently, the cells were cultured for an additional 72 h, after which their viability was assessed using a WST-1 assay kit (Roche Diagnostics, IN). .. Cell Viability Live/Dead Assay: For the live/dead assay, a portion of the aforementioned samples was subjected to further examination using calcein-AM and propidium iodide (PI) staining reagents obtained from the Double Staining kit (Dojindo, Japan). .. Subsequently, fluorescence microscopy (Nikon, Eclipse Ts2R) was employed to capture images of the stained samples.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma.
    Article Snippet: We used mito-TEMPO (S9733, Selleck chemicals, Houston, TX, USA) as ROS scavenger. .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15 nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 h after Actinonin administration.

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: Inhibition of glycolysis and stimulation of mitochondrial biogenesis lead to increased ROS levels and cell death in HNF-1ß positive clear cell carcinoma
    Article Snippet: We use si-GSK-3ß (D-003010-09, Horizon Discovery, Cambridge, UK) and si-control (D-001206-14, Horizon Discovery, Cambridge, UK). .. Double stain of viable or dead cells We utilized a double staining kit (CS01, Dojindo, Kumamoto, Japan) that contained Calcein-AM and Propidium Iodide (PI). .. Twenty-four hours after interfering with GSK-3ß (#D-003010-09, Horizon Discovery, CO, USA) (15nM) or control (#D-001206-14, Horizon Discovery, CO, USA), we administered Actinonin and collected cells, including the supernatant, at 54 hours after Actinonin administration.

    Cell Culture:

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 h and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Article Title: NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression
    Article Snippet: .. Cells were cultured in the glucose deprivation medium for 10 hours and then stained using a Double staining kit (Dojindo, 341-07381). ..

    Fluorescence:

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.

    Microscopy:

    Article Title: Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition.
    Article Snippet: Funding information Japan Agency for Medical Research and Development, Grant/Award Number: 19im0210221h0001 Abstract Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses.. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites.. This study demonstrated the application of a modified EPD technique utilizing Mg ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate.



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    Image Search Results


    Screening of genes in EC. (A) The Venn diagram shows the intersection between the DEGs in EC and normal esophageal tissues and the genes related to apoptosis, proliferation, and glycolysis. (B-D) The Lasso regression, SVM, and RF algorithms were used to further screen the 11 genes and identify key signature genes. (E) The Venn diagram shows the key genes identified by the Lasso regression, SVM, and RF algorithms.

    Journal: Regenerative Therapy

    Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

    doi: 10.1016/j.reth.2026.101101

    Figure Lengend Snippet: Screening of genes in EC. (A) The Venn diagram shows the intersection between the DEGs in EC and normal esophageal tissues and the genes related to apoptosis, proliferation, and glycolysis. (B-D) The Lasso regression, SVM, and RF algorithms were used to further screen the 11 genes and identify key signature genes. (E) The Venn diagram shows the key genes identified by the Lasso regression, SVM, and RF algorithms.

    Article Snippet: Apoptosis was evaluated using a Double Staining Apoptosis Kit (Vazyme Biotech, Nanjing, China) following the protocol for Annexin V-FITC and propidium iodide dual staining.

    Techniques:

    MMP12 knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis. KYSE150 cells were transfected with si-MMP12 or si-NC. (A and B) Cell migration and invasion were analyzed by transwell assays. (C) Cell proliferation was analyzed by EdU assay. (D) Cell apoptosis was assessed by flow cytometry. (E) HK1 and LDHA protein expression were detected by Western blotting. (F–H) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Journal: Regenerative Therapy

    Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

    doi: 10.1016/j.reth.2026.101101

    Figure Lengend Snippet: MMP12 knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis. KYSE150 cells were transfected with si-MMP12 or si-NC. (A and B) Cell migration and invasion were analyzed by transwell assays. (C) Cell proliferation was analyzed by EdU assay. (D) Cell apoptosis was assessed by flow cytometry. (E) HK1 and LDHA protein expression were detected by Western blotting. (F–H) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Article Snippet: Apoptosis was evaluated using a Double Staining Apoptosis Kit (Vazyme Biotech, Nanjing, China) following the protocol for Annexin V-FITC and propidium iodide dual staining.

    Techniques: Knockdown, Migration, Transfection, EdU Assay, Flow Cytometry, Expressing, Western Blot

    WTAP knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis by regulating MMP12 expression. KYSE150 cells were transfected with si-WTAP, MMP12 overexpression plasmid, or the matched control (si-NC and oe-NC). (A) MMP12 protein expression was detected by Western blotting. (B and C) Cell migration and invasion were analyzed by transwell assays. (D and E) Cell proliferation was analyzed by EdU assay. (F) Cell apoptosis was assessed by flow cytometry. (G and H) HK1 and LDHA protein expression were detected by Western blotting. (I–K) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ns: not significant, ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Journal: Regenerative Therapy

    Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

    doi: 10.1016/j.reth.2026.101101

    Figure Lengend Snippet: WTAP knockdown inhibited the migration, invasion, proliferation, and glucose metabolism and induced cell apoptosis by regulating MMP12 expression. KYSE150 cells were transfected with si-WTAP, MMP12 overexpression plasmid, or the matched control (si-NC and oe-NC). (A) MMP12 protein expression was detected by Western blotting. (B and C) Cell migration and invasion were analyzed by transwell assays. (D and E) Cell proliferation was analyzed by EdU assay. (F) Cell apoptosis was assessed by flow cytometry. (G and H) HK1 and LDHA protein expression were detected by Western blotting. (I–K) Glucose consumption, lactate production, and ATP levels were analyzed by commercial kits. ns: not significant, ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Article Snippet: Apoptosis was evaluated using a Double Staining Apoptosis Kit (Vazyme Biotech, Nanjing, China) following the protocol for Annexin V-FITC and propidium iodide dual staining.

    Techniques: Knockdown, Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, Control, Western Blot, EdU Assay, Flow Cytometry

    The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

    Journal: Bioactive Materials

    Article Title: Microenvironment-responsive injectable dynamic hydrogel for sequential antioxidant and tissue regeneration therapy of radiation-induced skin injury

    doi: 10.1016/j.bioactmat.2026.03.057

    Figure Lengend Snippet: The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

    Article Snippet: The Live/Dead Cell Double Staining Kit was supplied by Jiangsu KeyGEN BioTECH Co., Ltd., and the Cell Counting Kit-8 (CCK-8) was obtained from Beijing Solarbio Science & Technology Co., Ltd.

    Techniques: Activity Assay, Incubation, Staining, Co-Culture Assay